Process of producing peptone.



UNITED STATES P TENT ()EErcE.

MAX AUGUST SIEGFRIED, OF LEIPSIO, GERMANY, ASSIGNOR TO THE CHE- MISCHE FABRIK VON IIEY'DEN, AOTIEN-GESELLSCHAFT, OF RADEBEUL, GERMANY.

PROCESS OF PRODUCING PEPTONE.

SPECIFICATION forming part of Letters Patent No. 673,092. dated April 30,1901.

Application filed September 5, 1900. Serial No. 29,049. ($peci1nens.)

T whom it y 0071/0677 stanceswhich will precipitatelimeor baryta Beitknown that LMAXAUGUSTSIEGFRIED, for example, sulfuric or carbonic acid. The professor, a subject of the King of Saxony, solution .is then evaporated down at a low and a resident of Lindenstrasse l, Leipsic, temperature, best in oacu'o, or concentrated 5 Kingdom of Saxony, German Empire, have to a syrup and precipitated by alcohol, either 55 invented a new and useful Improvement in direct or after acidification, with acetic acid, the Method of Producing leptone, of which for example. From the filtrate from the pepthe following is a specification. tone-iron precipitate a further quantity of For the prod uction'of pure peptoneit is necpeptone-iron precipitate is obtained by fur- 1o essary that other decomposition products of ther addition of iron salt and neutralizing 6o albumen, the albumoses and products of dedown of the acid, for example, with ammonia, composition of the peptone, such as leucine, the fresh precipitate thus obtained being furshould be removed from the crude solutions ther worked up in the same way as the first, containing the peptone. The removal of the or the acid is at once neutralized down in the albumoses is not completely effected by saltproduction of the first iron precipitate, a bet- 65 ing out with ammonia sulfate, zinc sulfate, ter yield being thus obtained than wouldbe &c., in neutral alkaline and acid solutions, as obtained without total neutralization. hitherto practiced. Now this difificulty is Example I. Five hundred grams albumen overcome by myinvention,accor ding to which are digested in three liters 0.3 per cent. soda I add concentrated sulfuric acid or a satus0lution,with addition ofacommercialtrypsin 70 rated solution of an albumose-precipitating preparation. The solution is saturated with salt, such as ammonia sulfate or zinc sulfate ammonia sulfate by adding this salt in the and concentrated sulfuric acid, to the filtrate solid state and is then filtered; Concentrated containing peptone in a solution saturated sulfuric acid and saturated ammonia-sulfate with such a salt obtained by the hithertosolution are then added to the filtrate until 75 known methods. I add sulfuric acid so long no cloudiness occurs,even after standing some as any cloudiness occurs on long standing of hours. It is not possible to mention the prethe solution, or, according to my invention, cise quantity of the concentrated sulfuric at first only the greater proportion of the alacid to be added, as it depends on the quan- 0 bumoses may be precipitated by addition of tity of the albumoses and other secondary 8o concentrated sulfuric acid, a saturated solusubstances present in the mixture to be treattion of such an albumose-precipitating salt, ed. At all events the sulfuric acid is not insufficient for the complete precipitation of only added up to neutralization, but in avery the albumoses, being in this case added and considerable excess. Sulfuric acid is added 3 5 the remainder of the albumoses being reto the already strongly-acid liquid in such 85 moved by the addition of a ferric salt in quantitythatnofurtherturbidity takes place. strongly-acid solution. The albumose-free In the filtered liquid, after this has been neufiltrate obtained as above described is neutralized down with ammonia to a point at tralized down until it possesses afeebly-acid which it still gives a distinct acid reaction,

reaction andaferric salt, for example. Ironeighty grams iron ammonia alum are dis- 9o ammonia alum is then added thereto. The solved while stirring, the resulting iron preresulting iron precipitate is filtered OE and cipitate is filtered off, washed with saturated Washed with the saturated solution of the solution of ammonia sulfate, and stirred in saltforexample,ammoniasulfateused bewater with the addition of some ammonia.

fore for precipitating the albumoses. The The iron and sulfuric acid are separated by 95 peptone-iron salt obtained is treated with lime barium hydrate, the solution filtered, and the or its equivalent (baryta) after it has been excess baryta in the filtrate is precipitated by preferably stirred in water with addition of carbonic acid. The filtrate from the barium ammonia orsulfuric acid. The lime or baryta carbonate is evaporated in vacuo, the residue is separated from the-filtrate by means of subis dissolved in dilute acetic acid, and precipi- 10o tated by alcohol. Into the filtrate obtained from the iron precipitate, as above described, a further five hundred grams of iron-ammonia alum are stirred and the acid is totally neutralized down by gradual addition of ammonia. The resulting peptone-iron precipi- Eiate is further treated just the same as the rst.

Example II. Five hundred grams albumen are digested in three liters 0.3 per cent. soda solution with addition of a commercial trypsin preparation. The solution is saturated with ammonia sulfate by adding this salt in the solid state and is then filtered. Concentrated sulfuric acid is added to the filtrate until cloudiness no longer appears, even after standing some hours. To the filtrate, to which some ammonia has been added, but which is still strongly acid, are added eighty grams iron-ammonia alum and the whole is stirred. Into the filtrate from the resulting precipitate are stirred five hundred grams of iron-ammonia alum, and the acid is neutralized down by gradual addition of ammonia. The resulting iron precipitate is worked up, as in Example I, to peptone.

It is obvious that the method or process hereinbefore described is not limited to a process including the preparation of the crude peptone solution itself, as described in the above example. Peptone solution from any desired source may be subjected to the process. It will be further understood that the examples above given are merely typical of r the invention and that this is not restricted to the exact particulars and details therein described, which may be varied.

The peptone thus obtained is a white powder which is non-deliquescent, free from ash, and free from albumoses and other products of digestive action. It is not bitter, but possesses an agreeable taste. It has the pro nounced character of an acid and forms metallic salts.

I claim- 1. The method or process of producing peptone, consisting in saturating a crude peptone solution with an albumose-precipitating salt, precipitating the rest of albumose from the filtered solution by means of sulfuric acid and a concentrated solution of such a salt, filtering off the resulting solution, neutralizing the filtrate, precipitating a peptone-iron compound therefrom by means of a ferric salt, freeing the peptone from said precipitate by means of an earthy alkali, precipitating the earthy alkali from the resulting solution by a suitable agent and finally evaporating the solution at a low temperature, substantially as described.

2. The method or process of producing peptone, consisting in saturating a crude peptone solution with an albumose-precipitating salt, mixing the resulting solution with concentrated sulfuric acid and a ferric salt, filtering off the resulting solution, neutralizing the fil trate, precipitating a peptone-iron compound by means of a ferric salt, freeing the peptone from said precipitate by means of an earthy alkali, precipitating the earthy alkali from the resulting solution by a suitable agent and finally evaporating the solution at a low temperature, substantially as described.

In testimony that I claim the foregoing as my invention I have signed my name, in presence of two Witnesses, this 21st day of August, 1900.

MAX AUGUST SIEGFRIED.

Witnesses:

PAUL WILHELM SIEGFRIED, FRITZ KiiHNs. 

